If your ports are going through a PCI card I guess its possible that the card could have been fried (burnt out), although this is unlilnkely from a simple plugging in of a digital picture frame. Do you leave your PC running, or use lots of USB hubs with extensions; if so this shortens the life of your USB devices.
As I say it is unlikely however if you know much about computers I would advise taking a look inside the case and checking the PCI card isn't fried - if all else fails, take it to a store and they should just check it for free (and if you need a new card then you can buy it, which you would have to anyway even if you did open the case up yourself :D)
Alternatively you may have some how disabled something in the BIOS (not nescisarily the USB, maybe something powering or supporting the USB) and ensuring that everything looks correct within the "Device Manager"
Hope this helps!
Get out your voltmeter and check for 5 Volts between pins 1 and 4 ( the two outside pins ) of any USB connector on your computer. If you don't see 5 volts you have somehow blown the fusible resistor on your motherboard or USB adapter card. Obviously replacing it is not for the faint of heart.
Another way of checking is to try one of those dumb USB lights or fans. Make sure it works on a known good computer, then try it on yours. No light or fan - no 5 Volt supply. It's a relatively easy repair - for an expert.
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