I have a Lexus RX300 that won't lock or unlock via the door's auto switch on the driver side (it will also not unlock/lock via the key switch either)

I have read this 6 times and still not sure what the issue is? I have a 2005 ES330. To open all the doors with the key fob, you have to double press the Unlock Button. Pressing the Unlock button once, only opens the driver door.

The door lock module and further help is in the link.

https://www.google.com/search?q=2003+lexus+rx300.+door+lock+module+location

The Club Lexus forums are a great resource.

RX 300 Power Door Locks problem

ClubLexus https://www.clublexus.com > rx-1st-gen-1999-2003
Jun 24, 2008 - Door lock actuator relay/motor seems likely culprit. It has been discussed in the past. Try the search function. Although a different model, I ...

Power door lock switch do not unlock/lock driver's door.
Aug 1, 2007
03 RX300: Front driver side door lock not working - ClubLexus
Aug 12, 2010
Power door lock relay location? - Lexus Forum Discussion
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RX300 door locks intermittent - Lexus Forum Discussion
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More results from www.clublexus.com

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Check you haven't locked the rear window switches via the switch on the driver door.

May need new actuator. Try a slight lubricant like wd40 if that does not work. Try working switch back and forth. They usually start having problems when they get this old. You can have it replaced, but toyota /lexus is notorious for labor intensive repairs with can be costly if you take it to a dealer or very time consuming if you replace the actuator yourself.

Unplug window motor connector and test for voltage at the plug, while operating window switch. The age of the car warrants the window switch replacement (2000)

Hi!!
I believe your alarm is in the PASSIVE MODE, here is how it works.

PASSIVE MODE Setting (Arming) Anti-Theft System The system will automatically set 30 seconds after following methods are performed: Remove ignition key from ignition switch. Exit vehicle, and close all doors. After performing this, anti-theft indicator on left side of instrument panel will light continuously for about 30 seconds. After 30 seconds, anti-theft indicator will blink once per second. When system is set, doors cannot be locked or unlocked using door lock control switch.
CANCELING THE ALARM AND REPROGRAMING MAY SOLVE YOUR PROBLEM.
Canceling Anti-Theft System Setting (No Alarm) When system is set (armed), but alarm is not sounding, use one of following methods to cancel system setting:

• Unlock left or right front door using key.
• Unlock all doors using remote keyless entry system.
• Insert key in ignition switch and turn to ON position (this is operative only when anti-theft system has not been triggered).

HERE IS HOW TO REPROGRAM THE ALARM IN CASE YOU NEED TO RESET IT.
PROGRAMMING
PASSIVE MODE NOTE:Initial mode is passive mode off. If there is a different signal in middle of programming, signal is invalid.
Programming Passive Mode Off

• Remove ignition key from ignition key cylinder. Ensure all doors (including back door) and engine hood are closed and unlocked. Perform all of following methods within 30 seconds:
• Lock or unlock any door with key or remote keyless entry system.
• Open driver door.
• Lock and unlock driver door lock knob 3 times.
• After performing this, system should start force lock after 2 seconds. Perform all of following methods within 20 seconds:
• Unlock driver door lock knob.
• Close and open driver door 2 times.
• Lock and unlock driver door lock knob.
• After performing this, system should start force lock after 2 seconds. Passive mode should now be off.

Programming Passive Mode On

• Remove ignition key from key cylinder. Ensure all doors, engine hood and luggage compartment door are closed and unlocked. Perform all of following methods within 30 seconds:
• Lock or unlock any door with key or remote keyless entry system.
• Open driver door.
• Lock and unlock driver door lock knob 3 times.
• After performing this, system should start force lock after 2 seconds. Perform all of following methods within 20 seconds:
• Unlock driver door lock knob.
• Close and open driver door 4 times.
• Lock and unlock driver door lock knob.
• After performing this, system should start force lock after 2 seconds. Passive mode should now be on.

If you need further assistance, please provide an email address to send more info on your security system
Good Luck!! A 4 THUMBS UP rating would be greatly appreciated for this solution. Thank you for using FixYa!!

https://www.clublexus.com/forums/rx-1st-gen-1999-2003/395765-driver-door-won-t-lock-or-unlock-using-key-transmitter.html

Check your starter solenoid or your ignition switch, sounds like there is a short causing the car to start when the weather changes

Change your battery first, then try again. The codes don`t normally erase on their own.
I had a new master key programed this week and watched the process at the dealership. Mine was done with the same diagnostic code reader they use for the entire vehicle, attached under dash via cable,with the master key along side it is like a giant meter. It took about 7 minutes

1. sitting in driver seat, with driver's door OPEN, press lock/unlock button ON THE DRIVERS DOOR to lock all doors, then manually unlock driver door only.
2. Insert YOUR key in ignition, DO NOT TURN, then remove key.
3. Using the same lock/unlock button ON THE DRIVERS DOOR, slowly press lock-unlock in 5 cycles
4. Shut door.
5. Open door.
6. Using the same lock/unlock button ON THE DRIVERS DOOR, again slowly press lock-unlock in 5 cycles
7. With the door open insert your key into the ignition, turn to 'on' position (DON'T START), wait 5 seconds. Turn off and remove key.
8. Locks should cycle once (Car will lock and then unlock once by herself). If they don't cycle or they cycle more than once, do steps 1-7 over.
9. Now using the remote, push lock button, hold for one second, then release the button. The locks should cycle once. If not, start over and go slower.
10. Close the door.
11. Open the door.
12. Press the lock button on the remote. The locks should do a lock-unlock cycle.
13. Close the door
14. Press the lock button on the remote, the doors should lock.
15. Press unlock button and the doors should unlock.

Long beep to follow if successful.