2001 f250 super duty 5.4 4x4 Transmission worked fine yesterday morning. left in truck same evening about 3 miles up road hit ruff place in road and truck slowed to about 10 miles per hr. overdrive light went to blinking. turned around an headed back home and found in first and second gear it seem to be ok but in drive would only go about 10 miles per hr. unhooked battery this morning for about 30 minutes and now truck wont hardly move at all.

Hello and welcome to FixYa!
As a first course of action, I strongly suggest that you replace the transmission fluid. If the fluid is burnt issues like this occurs. Once the transmission fluid is replaced the transmission can work normally again.
If the problem still persists after the transmission fluid replacement then the fault can be on the TCM (transmission control module). The TCM is like a brain of the transmission that when it is faulty it won't engage in gears or it may engage in gear but won't move at all. If that's the case, you will need a TCM replacement to resolve the problem.
Hope this helps and thank you for using FixYa! Have a good one!