Whether the bolts are nylon or brass, they all come loose after a while. The culprit is oversize holes in the china bowl. To solve the problem, go to the auto parts store and buy a short length of fuel hose, just large enough to slip over the toilet seat bolt. Cut two short pieces of hose off -- about 1/4 inch each -- with a sharp bread knife or utility knife. Fit the pieces of hose into the toilet seat mounting holes in the bowl. If the hose is too large, snip a small segment out of it to make it into a "C" shape, just large enough that the cut ends touch when inserted into the mounting holes. Install the seat as usual and snug up the nuts.
Once movement of the seat is prevented, the bolts tend to stay tight. I've performed this trick on a number of my own and friends' toilet seats and they've stayed put for 8-10 years with no signs of loosening, yet can be removed easily for maintenance and cleaning.
Use fuel hose, as it is durable and does not deteriorate with exposure to air. This trick won't work with electrical tape, duct tape or masking tape, as those materials are too soft and the adhesive cold-flows under pressure.
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