Rear cylinder not firing
Carb or fuel injection? Spark or no spark? Check for spark by putting a new plug into lead and laying on cylinder head. Leave old plug in engine. Start bike. If carb and no spark then plug or coil would be first suspects, then ignition module, then wiring from coil to ignition module. This is assuming you have compression on rear cylinder and all mechanical checks completed. If fuel injection and good spark, then ECU most common problem. Then injector, then wiring. If no spark, check coil, then wiring frpm coil to ECU, then ECU.
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