My 2001 wide glide has no spark What is the first thing I should check

Will engine turn over? Are you getting spark to the plugs?

Pull out the filler plug and it has a dipstick on it.

Hi ,maybe you can read this article ,it can help you.How to replace spark plug and coil ,good luck!

Check the leads to your spark plugs. These wires are cheap to change out and have a reputation for intermittent failure

Check that out with an H-D paqrts department.

I'd take the plug out of the case and check voltage with a multi meter

Hi, Anonymous and the usual suspects are:
1. Fouled spark plugs.
2. Severely discharged or a damaged battery should have 12.5 volts or more and be able to pass a proper "LOAD" test if necessary, you may have a preliminary reading of 12.5 volts or more but little or zero amperage the battery is faulty and must be replaced, AGM batteries fail in this scenario more so than lead-acid batteries.
3. Check battery terminals for damage or corrosion, check the battery cables at "BOTH" ends for loose, corroded, or broken connectors, "INSIDE" and outside the cable harness, perform connector wiggle test and check cables with an ohmmeter if necessary.
4. Loose connection at ignition coil or plug between ignition sensor and module.
5. Spark plug cables in bad condition, shorting/leaking, spark plug cable connections loose check for spark leakage in the dark.
6. A faulty ignition coil or electronic control module.
7. Faulty pulse coil.
8. Faulty CKP, CMP, or BAS sensor.
9. Faulty ignition switch.
10. Faulty run/off switch
11. Tilt sensor needs a reset.
12. Security alarm failing to disarm needs reset
For more information about your issue and valuable "FREE" downloads that you will need for viewing or printing please click on the blue links below. Good luck and have a wonderful day.
https://www.hdforums.com/forum/dyna-glide-models/1037786-2000-dyna-no-spark-won-t-start.html
http://www.mediafire.com/file/g3h2vn0nvw3b5wb/2001_Harley_Dyna_Service_Manual.pdf/file
https://partsfinder.onlinemicrofiche.com/ronnies/showmodel.asp?make=hdmc
https://serviceinfo.harley-davidson.com/sip/service/document/6300
https://www.6ya.com/how-6ya-works to speak to me personally.

try new spark plugs or check your coil to see if its sparking if not try cleaning the earth of the coil if that doesn't work try a new coil

.38 - .42 ........i use a wire gauge and set it right at .40

Have your alternator checked.