A slide bar down under back edge of a back window that keeps it open on a 2005 Highlander? What is that?

Start the vehicle,hit the up button on that door JUST AS you give the door a hard WHACK.It may take more than one try but the window should go back up.

Most likely, you missed a hidden bolt. There aren't pins or clips that hold these on. If it is tight against the transmission, it is a bolt. If it slides back a bit and the gap is they same all the way around, then there is possibly a clip on the shaft inside of it. Pry the T case off. Caution though. If you can pry it open some, But it will not stay or it won't open the same all way around, you missed a bolt. Do not pry it loose, you will break the T case housing.

I can't think of any reason why it would. It's just an interior panel. Watch what you are doing and it should be fine

You need to see if the latch bar ( curved metal piece that holds onto the strike bolt ) is in the closed position - if so, you'll need a helper to open it. You should also check to see if it is lubricated, which can keep it from latching.
When you look into the latch assembly, you should see a clear pathway for the striker bolt to enter the assembly. If there is a bar ( or hook ) blocking it, then you can use a flat blade screwdriver to move it to the open position. Insert the screwdriver into the latch assembly where you can pry on the inside of the hook, using the body of the door for leverage. position your screwdriver and then have your helper pull ( or push ) the outside door button / handle as you apply a small amount of pressure to the inside of the hook - the hook should easily slide out of the way. If it is hard to move, you'll need to spray some lubrication into the latch assembly. You can buy a can of white lithium grease in a spray can that will do the job.
There is a certain angle you'll need to pry in to get the latch to open - you may need to place the blade of the screwdriver through the hook and on the outside edge of the door or outside edge of the latch assembly, then gently push the screwdriver handle towards the outside of the van as the handle/ button is used.
Hope this helps!!

You don't mention HOW she "puts the window back up" - does the power window work OK when raising or lowering the window.
There may a short circuit causing the window motor to turn on.
Let me know if the window operates normally sometimes; and whatever information or description you might have.

i just went threw that with my gf,s 05 highlander. auto zone said it was gas cap. i changed the air filter n it came back on then i took the little filter on top of air filter out with a pry bar out and reset it by taking batt post off 15 min n its been off awhile now. "filter is on top of air filter in the top part of air box".

Not a car expert but have a lot of experience working on my cars over 30 years.

Just a few suggestions---if rear only and vehicle has safety child locks on rear doors (a slide bar down under back edge) that will stop windows in rear from opening no matter what control is used.

Another common issue I have had is the master button panel on driver door can fail partly--power goes there and then to rear windows---can be tested but usually have to replace entire button unit to know.

A relay is remotely possible but not likely unless they are in the door and two went bad.

Most vehicles I have seen have only one fuse or power source for all four or more windows.

Again, not a expert in this area but saw this and figured some advice is better than no advise.

The child lock feature for rear doors usually disables the windows as well and is easy to miss.

If all your other windows are working it must be a loose connector at the power window regulator. You have to remove the door trim and check the connector. If there is a water leak it could have cause damage to the window regulator itself or cause corrosion in the connector

if it makes a grinding or poping noise it will most likely need new sunroof track cables

You should have hit the dealer up for some of the cost as this vehicle was only just out of warranty