Went to get in my car this morning and remotes would not work at all. Tried to program garage door last night, they worked fine

You might have accidently turned on the timer settings.
Select manual option if it's on auto.

There is a computer that controls all those things. IT is called the BCM, it is bad. They are not cheap.

Electrical issue is causing your hassles. Look for a bad ground in circuit common to those items.

do you know if the remote is a after market or factory remote. have you tried to stick the key in the door lock and lock and unlock the door,and when start it. all new cars have have theif module in them ,and if is bypassed it will lock up

The 2.7 had alot of problems with the cam sensor. I would start there. Its located on the front top of the timing cover. Unplug it and see if it "Pops" attempts to start.

soinds like a short in the wireing or possibly burned out relay

If this model has a child safety lockout to keep children from unlocking passenger doors flip the switch back to turn the feature off

I think your problem has sorted itself. No need to go to garage.

There was a minor change for mine to work.. see below..

- you must park your Prius close to the gate opener, leaving it running (obviously not in the way of the gate closing)
- Hold the button the the mirror and then push your garage door remote button and hold until the light on the mirror starts to flasyh fast. THIS programs the button on the mirror, but not the garage opener - yet (this was key for me)
- locate the "learning button" on the opener itself
- press the "learning button" on your gate opener and then get back into the Prius within 30 seconds (more time than it sounds) and press the SAME Homelink button you just programmed above. I don't recall how long I held the pre-programmed button, but the light will flash as I recall.
- Then, when working the garage door, you may need to hold the mirror button for a good second for it to work - but I had tried for months to get mine to work, and finally after reading many posts, the above sequence worked on my 2008 Prius with my Lift Master, Security + rolling codes garage opener.

Just - program the button on the mirror with your remote FIRST.. the garage opener will not work.. then use the Learn button on the garage opener and the SAME BUTTON you just programmed.. and it worked great for me. Not programming the mirror button first will not work. Using only the Learn button will not work. You must program the mirror first with the remote, then use the learn button and the SAME button on the mirror. Hope this helps.

Scott 2008 Prius with Lift Master Security + opener.
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Its you antenna module above your headliner. It controlls your remote for keyless entry as well as your am/fm reception via an amplifier. GM part number is 12233252 for updated and revised version around $100 bucks online.